- DNA-constructs (Google spreadsheet)
- Addgene plasmids
- Links to plasmids with maps (powered by GenomeCompiler)
- Research Material (plasmids, primers, cells)
- Molarity Calculator
- Macros for ImageJ
Curve fitting with R: Example: R-Markdown-link
- New: dose-response curve (with curve fitting and R2)
- DNA-calculations
- Biorad Protein Assay
- BCA-Protein Assay (Harald Freudenthaler)Excel.jpg (272893 Byte)
- FLIP (fluorescence loss in photobleaching)
- FRAP (fluorescence recovery after photobleaching)
- template for single values
- template for 5 replicates + curve fitting ... NEW
(Solver Add-In has to be activated)
- template for 5 replicates
- template for single values with control region - FRET: correction factors, Youvan and Xia values
- Molarity calculations
- realtime PCR (quantitative PCR, qPCR: Link to Online Excel)
- with dilution curve to determine PCR effciency - realtime PCR with quantification of the PCR efficiency based on the amplification curve
- SDS-PAGE: MW-calculations
- Collection of standard methods (from Hannah Neumeier's diploma thesis)
- beta-Galactosidase CPRG plate reader assay
- Cell cycle analysis by PI-staining and flow cytometry
- Co-immunoprecipitation
- EMSA's (electrophoretic mobility shift assays)
- ISNT (In Situ Nick Translation for the morphological detection of apoptotic cells)
- JC1-staining to detect apoptotic cells (via the mitochondrial membrane potential)
- Kinase assays
- Northern Blots
- PCR-cloning
- SDS-PAGE buffer recipes
- Transfections with Calcium
- Transfections with Lipofectamine Plus
- Western Blots (for detection of myc, HA, flag, I-kappa B, GFP, TAK1)
- Yeast-DNA preparation