Maki TSUJITA
Junior Associate Professor
Department of Biochemistry
Nagoya City University
Graduate School of Medical Science
Mizuho-ku Nagoya 467-8601
JAPAN
Website Maki Tsujita
Programm
”Apolipoprotein A-I origin and the function of ABCA1 in mouse adult-neurogenesis at the hippocampal dentate gyrus”
ApoA-I and ABCA1 are the most effective components to generate HDL in the plasma. ApoA-I has been identified in the human CSF even though no generation is detected in neuronal cells or glial cells. We generated the apoA-I floxed mice and discovered liver- and intestinal- apoA-I are the origin of apoA-I in mouse CSF. To reveal the further function of HDL in the brain, adult-neurogenesis of ABCA1-null mice was examined. F-ara-EdU was injected peritoneally and after 28 days of breeding, newly generated mature neuronal cells were labeled by Alexa-488 using the Click Chemistry method. Positive cells at the granular zone and the sub-granular zone were counted. In wild-type mice, 1.6 cells per DG slice. On the other hand, 0.44 positive cells per DG slice (P=0.0001 vs control WT mice) were detected in Abca1-null mice. Interestingly, The WT mice which exposed to 0.4 T of magnetic field (for 2 h) showed 0.47 positive cells per DG slice (P = 0.003). These results suggest the disruption of HDL generation by deletion of ABCA1 and by magnetic field affects adult neurogenesis in mice.
Host: Thomas STOCKNER
Contact for questions: Helmut KUBISTA