Dr. Daniel BACHURSKI
Universitätsklinikum Köln
Website des Labors für molekulare Pathogenese der CLL

Program

"Advancing single-cell analyses of EV recipients with tellurium-based labeling of the extracellular vesicle proteome (TeLEV): Insights and Applications"

The field of extracellular vesicles (EVs) is evolving, demanding innovative techniques for detailed single-cell analysis of EV recipients. Our novel labeling method, tellurium-based labeling of the extracellular vesicle proteome, utilizes L-2-tellurienylalanine (TePhe), a tellurium-based phenylalanine analog, to mark EVs. This allows for precise identification of EV recipient cells through mass cytometry (MC) and imaging MC (IMC), capable of distinguishing over 52 heavy metal isotopes. Our approach, combining TeLEV with MC, offers unprecedented insights into EV uptake across a diverse range of immune cells and their subsequent functional changes, including apoptosis, DNA damage response, and various other signaling pathways. We validated TeLEV with multiple cell lines and primary cells, ensuring no alteration in EV composition or morphology compared to unmarked EVs. Our findings highlight the differential uptake of TeLEV-marked EVs by specific cell subsets, particularly monocytes and dendritic cells, with a notable absence in T cells. This research opens new possibilities for investigating EV distribution and their impact on recipient cells, leveraging the strengths of MC and IMC technologies.

Host: Ulrike RESCH

Contact for questions: Helmut KUBISTA